| 摘要: |
| 目的 了解髓过氧化物酶（MPO）-抗中性粒细胞胞质抗体相关性血管炎（AAV）患者血清中IgG4及辅助性T细胞（Th） 1/Th2细胞因子的表达情况。方法 收集苏州大学附属第二医院50例AAV患者及40例同期健康体检者的血清样本，采用免疫比浊法测定IgG水平，采用ELISA法测定IgG4水平，采用流式细胞术微球阵列（CBA）技术检测血清中Th1/Th2细胞因子的水平，比较两组样本上述指标的差异，分析不同指标之间的相关性。结果 共收集到50例AAV患者血清样本，其中MPO-AAV患者40例（80.0%）。MPO-AAV组血清IgG4水平为（1.13±0.33） g/L，IgG4/IgG比值为0.094±0.032，IL-4水平为（2.54±0.68） pg/mL，IL-6水平为15.22（8.83，59.52） pg/mL，均高于健康体检者［IgG4水平为（0.80±0.27） g/L，IgG4/IgG比值为0.070±0.028，IL-4水平为（1.96±0.60） pg/mL，IL-6水平为4.95（2.82，13.25） pg/mL］，差异均有统计学意义（P均＜0.01），且MPO-AAV患者血清IgG4水平与IL-6水平呈正相关（rs＝0.508，P＜0.05）。结论 我院AAV患者中MPO-AAV比例较高。MPO-AAV患者血清中IgG4水平及IL-4、IL-6水平均升高，且IgG4升高与IL-6升高呈正相关，提示IgG4的升高与MPO-AAV患者Th1/Th2细胞因子平衡失调有关。 |
| 关键词: 免疫球蛋白G4 免疫球蛋白G Th1/Th2细胞因子 抗中性粒细胞胞质抗体相关性血管炎 髓过氧化物酶 |
| DOI：10.16781/j.CN31-2187/R.20190458 |
| 投稿时间：2019-05-18修订日期：2021-03-31 |
| 基金项目: |
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| Detection and analysis of serum IgG4 and Th1/Th2 cytokines in patients with myeloperoxidase-anti-neutrophil cytoplasmic antibody-associated vasculitis |
| QIN Chen-hao,GU Ai-ping,ZHAN Fu-qin,FENG Ping,GU Hao-yu,DU Hong* |
(Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu, China
*Corresponding author) |
| Abstract: |
| Objective To investigate the expression of immunoglobulin (Ig) G4 and helper T cell (Th) 1/Th2 cytokines in the serum of patients with myeloperoxidase-anti-neutrophil cytoplasmic antibody-associated vasculitis (MPOAAV).Methods Serum samples of 50 AAV patients and 40 healthy controls who taking a physical examination in the same period were collected from The Second Affiliated Hospital of Soochow University. The levels of IgG and IgG4 were detected by immunoturbidimetry and enzyme-linked immunosorbent assay, respectively. The expression of Th1/Th2 cytokines in serum was detected by flow cytometry microsphere array (CBA). The differences of the above indexes between the 2 groups were observed, and the correlations between different indexes were analyzed.Results Serum samples were collected from all the 50 AAV patients, including 40 of MPO-AAV patients (80.0%). In the MPO-AAV group, the serum IgG4 level was (1.13±0.33) g/L, IgG4/IgG ratio was 0.094±0.032, IL-4 level was (2.54±0.68) pg/mL, and IL-6 level was 15.22 (8.83, 59.52) pg/mL, which were significantly higher than those in the control group (IgG4: ［ 0.80±0.27］ g/L, IgG4/IgG ratio: 0.070±0.028, IL-4: ［1.96±0.60］ pg/mL and IL-6: 4.95 ［2.82, 13.25］ pg/mL; all P＜0.01), and there was a positive correlation between the serum IgG4 and IL-6 levels in the MPO-AAV patients (rs＝0.508, P＜0.05).Conclusion The proportion of MPO-AAV patients is high in AAV patients in our hospital. The serum IgG4, IL-4 and IL-6 levels in MPO-AAV patients are significantly increased, and the level of IgG4 is positively correlated with the level of IL-6, suggesting that the increase of IgG4 is related to the imbalance of Th1/Th2 cytokines in MPO-AAV patients. |
| Key words: immunoglobulin G4 immunoglobulin G Th1/Th2 cytokines anti-neutrophil cytoplasmic antibody-associated vasculitis myeloperoxidase |
