The coronavirus disease 2019 (COVID-19) was reported in Dec. 2019. In Mar. 11, 2020 (local time), the World Health Organization (WHO) declared COVID-19 a pandemic. Preventing the healthcare-associated infection of COVID-19 is one of the key steps for controlling the source of infection, cutting off the route of transmission and eliminating the pandemic. We analyzed the current challenges and problems in the prevention and control of healthcare-associated infection of COVID-19, and proposed corresponding and targeted medical countermeasures for the prevention and control of COVID-19 can from the aspects of personnel, hardware and process management, which may contribute to better dealing with the pandemic challenge of COVID-19, protecting medical staff from occupational injuries and getting the pandemic under control.