Objective To establish a high-performance liquid chromatography (HPLC) method for simultaneously determining the two triterpenoid acids (schisandronic acid[SA] and coccinic acid[CA]) in Schisandra, and investigate their content differences in Schisandra from different habitats, so as to provide basis for improving the quality of medicinal materials. Methods The HPLC condition was as follows:Waters Symmetry C₁₈ column (4.6 mm×250 mm, 5 μm), the mobile phase was composed of methanol-0.1% formic acid solution (86:14), the detection wavelength was 220 nm, the flow rate was 1.0 mL/min, the column temperature was 30℃, and the injection volume was 10 μL. The contents of SA and CA were determined in Schisandra from Liaoning, Jilin, Hubei, Hunan, Shaanxi, Shanxi, and Henan. SPSS software (SPSSAU) was used to analyze the obtained data. Results There were good linear relationships in the range of 10-800 μg/mL for both SA and CA, the correlation coefficients were both 0.999 7, and the average recovery rates were 97.25% (RSD=2.04%, n=6) and 96.02% (RSD=2.03%, n=6), respectively. The contents of SA and CA in Schisandra from different habitats varied greatly. The contents of SA and CA in Schisandra sphenanthera (from Hubei, Hunan, Shaanxi, Shanxi, and Henan) were higher than those in Schisandra chinensis (from Liaoning and Jilin). The highest content of SA in Schisandra was from Henan ([0.285±0.015] mg/g) and the lowest one was from Jilin ([0.068±0.017] mg/g. The highest content of CA was from Shanxi ([0.927±0.017] mg/g) and the lowest one was from Jilin ([0.039±0.010] mg/g). Conclusion The method in this study is simple, accurate, reliable and reproducible, and it is suitable for the simultaneous quantitative analysis of the two triterpenoid acids SA and CA.