Objective To analyze the effect of standard stroke treatment procedures of Changhai Hospital on treatment time of patients with acute ischemic stroke (AIS) caused by large vessel occlusion.Methods A total of 876 consecutive patients with large vessel occlusion AIS who underwent endovascular treatment in Neurovascular Center of our hospital from Sep. 10, 2013 to Dec. 31, 2019 were retrospectively selected; 66 patients without complete data were excluded and 810 were finally included. The patients who received the standard stroke treatment procedures (implemented from Jun. 22, 2018) were assigned to study group (n=335), and the patients who did not received were assigned to control group (n=475). The door-to-needle time (DNT), door-to-puncture time (DPT), puncture-to-recanalization time (PRT), successful recanalization (modified thrombolysis in cerebral infarction grade ≥ 2b) rate and good prognosis (modified Rankin scale score ≤ 2) rate 90 d after operation were compared between the 2 groups.Results There were no significant differences in gender, age or National Institutes of Health stroke scale score on admission between the 2 groups (all P > 0.05). The proportion of patients treated with intravenous thrombolysis+endovascular treatment was significantly lower in the study group than that in the control group (14.3%[48/335] vs 31.2%[148/475], P < 0.001). In the patients treated with intravenous thrombolysis+endovascular treatment, the DNT, DPT and PRT were significantly shorter in the study group than those in the control group (34.2[28.1, 60.4] min vs 53.5[27.0, 72.2] min, 76.5[55.9, 106.4] min vs 97.0[68.9, 151.1] min, and 45.0[37.3, 90.4] min vs 78.0[55.4, 109.3] min, all P < 0.01). There were no significant differences in the good prognosis rate 90 d after operation or successful recanalization rate between the 2 groups (66.7%[32/48] vs 57.4%[85/148] and 91.7%[44/48] vs 93.2%[138/148], both P > 0.05). In the patients treated with endovascular treatment alone, the DPT and PRT were significantly shorter in the study group than those in the control group (67.0[50.1, 109.0] min vs 87.0[60.8, 150.0] min and 48.0[43.5, 80.8] min vs 74.0[60.3, 100.6] min, both P < 0.001). There were no significant differences in the good prognosis rate 90 d after operation or successful recanalization rate between the 2 groups (54.7%[157/287] vs 52.3%[171/327] and 93.0%[267/287] vs 91.1%[298/327], both P > 0.05).Conclusion The standard stroke treatment procedures in our hospital can significantly shorten the DNT, DPT and PRT in treating the patients with large vessel occlusion AIS, without reducing the successful recanalization rate and 90 d good prognosis rate.