| 摘要: |
| 目的：观察低温干预对沙土鼠单侧脑缺血后氨基酸递质的影响。方法：16只蒙古沙土鼠随机分为常温［直肠温度(37.0±0.2)℃］和低温［直肠温度(32.0±0.2)℃］组。右侧颈内动脉钳夹30 min，用脑内微透析和高效液相色谱法测定右侧海马CA1区胞外氨基酸递质含量。结果：低温明显抑制了细胞外谷氨酸、甘氨酸浓度的升高，使兴奋毒性指数显著降低。结论：低温对脑缺血的保护机制之一可能是降低了神经细胞的兴奋毒性指数。 |
| 关键词: 低温 脑缺血 氨基酸递质 微透析 |
| DOI： |
|
| 基金项目: |
|
| The protective effect of hypothermia on unilateral cerebral ischemia in gerbils |
| 朱兴族,王晓雪,菝??张社卿 |
| () |
| Abstract: |
| Objective:To investigate the effect of hypothermia on amino acid neurotransmitters in gerbil models of unilateral cerebral ischemia.Methods:Sixteen Mongolian gerbils were randomly subjected to unilateral internal carotid artery occlusion under conditions of either 37℃ or 32℃ for 30 min.Hippocampal concentrations of glutamate ,glycine and GABA were monitered using in vivo microdialysis combined with high performance liquid chromatography.Results:Hypothermia significantly inhibited the release of glutamate and glycine,therefore the high excitotoxic index seen in the normothermic group was strikingly decreased in the hypothermic group.Conclusion:The neuroprotective effect of hypothermia on cerebral ischemia may reside , in part , in its ability to decrease extracellular excitotoxic indexes. |
| Key words: hypothermia cerebral ischemia amino acid neurotransmitters microdialysis |
