| 摘要: |
| 目的：观察促性腺激素释放激素(LHRH)类似物那法瑞林对体外培养的人乳腺癌细胞BCaP-37生长的影响。方法：培养的BCaP-37细胞，经下列条件作用4 d后进行细胞计数：(1) 加入10-5-10-9mol/L的那法瑞林；(2)加入10-7-10-10mol/L的雌二醇(E2)；(3)在含10-8mol/L E2的条件下，加入10-6-10-8mol/L的那法瑞林或他莫昔芬；(4)加入10ng/ml或50ng/ml的表皮生长因子(EGF)；(5)在含10ng/ml EGF的条件下，加入10-5-10-7mol/L的那法瑞林。结果：那法瑞林在10-5-10-9mol/L浓度下对BCaP-37培养细胞的生长无显著抑制作用，在10-6-10-8mol/L浓度下可拮抗10-8mol/L E2对BCaP-37细胞的生长促进作用，对10ng/ml EGF抑制BCaP-37细胞生长的作用无显著影响。他莫昔芬拮抗E2促进BCaP-37生长的作用与那法瑞林相似。结论：那法瑞林对人乳腺癌细胞BCaP-37体外培养生长无显著抑制作用，但能拮抗E2对BCaP-37细胞的生长促进作用。 |
| 关键词: 促性腺激素类 乳腺肿瘤 那法瑞林 |
| DOI： |
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| Inhibitory effects of LHRH analogue nafarelin on the growth of BCaP-37 human breast cancer cells in vitro |
| 褚云鸿,顾军,周颖奇 |
| () |
| Abstract: |
| Objectve: To study the effects of luteinizing hormone-releasing hormone (LHRH) analogue nafarelin, estradiol(E2) and tamoxifen on the growth of BCaP-37 human breast cancer cell line in vitro. Methods: BCaP-37 human breast cancer cells (2.5×104/well) were cultured in DMEM with 5%no estradiol bovine serum. The number of BCaP-37 cell was counted after 96 h culture in following conditions respectively: (1) with nafarelin at 10-5-10-9mol/L, (2) with E2 at 10-7-10-10mol/L, (3) with nafarelin or tamoxifen at 10-6-10-8mol/L stimulated by 10-8mol/L E2, (4) with epidermal growth factor (EGF) at 10ng/ml or 50ng/ml, (5) with nafarelin between 10-5mol/L and 10-7mol/L influenced by EGF at 10ng/ml. Results: Nafarelin had no inhibitory effect on growth of BCaP-37 cell cultured at 10-5-10-9mol/L. At the concentrations between 10-6and 10-8mol/L, nafarelin or tamoxifen could antagonize the cell growth stimulated by E2 (10-8mol/L) respectively, and nafarelin had no effect on growth of BCaP-37 cell inhibited by EGF (10ng/ml). Conclusion: Nafarelin has no inhibitory effect on growth of BCaP-37 human breast cancer cell in vitro, but can antagonize the cell growth stimulated by estradiol. |
| Key words: gonadotropins breast neoplasms nafarelin |
