| 摘要: |
| 目的： 观察体外TNFα联用IL-2培养LAK细胞的抗胃癌作用及TNFα预处理后胃癌细胞对LAK细胞敏感性的变化。方法: 以不同浓度TNFα与IL-2配伍培养LAK细胞, 并做TNFα的单抗阻断试验, 用LDH释放法检测LAK细胞抗癌活性;以TNFα预处理胃癌细胞, 检测LAK细胞抗瘤活性。结果: TNFα在低浓度IL-2下增加LAK活性,使IL-2诱导同等LAK活性的浓度下降约10倍;TNFα预处理使LAK对其抗瘤活性提高约9％。结论: TNFα可增加IL-2诱导的LAK抗胃癌活性；TNFα预处理可使胃癌细胞对LAK的敏感性增加。 |
| 关键词: 肿瘤坏死因子 胃肿瘤 杀伤细胞，淋巴因子激活 |
| DOI： |
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| 基金项目: |
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| Influences of TNFα on effectiveness of LAK cells on gastric cancer |
| 吴裕忻,章永平,张兴荣,刘苏 |
| () |
| Abstract: |
| Objective: To investigate the anti-gastric cancer (GC) effect of LAK cells induced by TNFα with IL-2 and the influences of pretreatment with TNFα on the susceptibility of GC cells to LAK. Methods: Induced by TNFα combined with IL-2 at different concentrations, blocked by TNFα McAb, and after pretreating the GC cells with TNFα, LAK cytotoxicity to GC cells was detected by LDH colometric assay. Results: At low concentrations of IL-2, TNFα increased the cytotoxicity of LAK and the concentration of IL-2 necessary to induce the cytotoxicity to the same extent decreased 10 folds. Pretreatment of GC cells with TNFα made the cytotoxicity of LAK to the GC increase by 9%. Conclusion: TNFα increased the cytotoxicity of LAK induced by IL-2 to GC cells, and the GC cells pretreated with TNFα were more susceptible to LAK. |
| Key words: tumor necrosis factor stomach neoplasms killer cells, lymphokine-activated |
