| 摘要: |
| 目的：研究黄颜木素对激活的永生型大鼠肝储脂细胞--HSC-T6细胞增殖和胶原合成的影响。方法：细胞增殖采用结晶紫染色法检测，胶原合成采用3H-脯氨酸掺入法分析。结果：黄颜木素（6.25-50.00μmol/L）以剂量依赖方式显著抑制血小板源生长因子(PDGF)刺激的HSC-T6细胞增殖,并抑制转化生长因子β1(TGFβ1)诱导的细胞内胶原合成。结论：黄颜木素具有抑制激活的肝储脂细胞增殖和胶原合成的作用。 |
| 关键词: 黄颜木素 血小板源生长因子 转化生长因子β 肝硬化 |
| DOI： |
|
| 基金项目: |
|
| Effects of fisetin on proliferation and collagen synthesis of HSC-T6 cells |
| 钱定华,王杰松,张俊平,张珉 |
| () |
| Abstract: |
| Objective: To study the effects of fisetin on proliferation and collagen synthesis of immortalized rat hepatic stellate cell line：HSC-T6 cells . Methods: Cell proliferation was measured by crystal violet stain ing assay. Collagen synthesis was determined by 3H-proline incorporation. Results: Fisetin (6.25-50.00 μmol/L)markly inhibited PDGF-stim ulat ed proliferation and TGFβ1-stimulated collagen synthesis of HSC-T6 cells in a concentration-dependent manner. Conclusion: Fisetin could inhi bit actived hepatic lipocyte proliferation and collagen synthesis |
| Key words: fisetin platelet-derived growth factor transforming growth f actor beta liver cirrhosis |
