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  • 胡以平,郝光荣,孙伟,王新民,李建秀,戴德顺,胡卫江.乙型肝炎病毒纯合子转基因小鼠(adr 1.2)的培育[J].第二军医大学学报,1999,20(9):    [点击复制]
  • 胡以平,郝光荣,孙伟,王新民,李建秀,戴德顺,胡卫江.The generation of hepatitis B virus transgenic mice homozygote[J].Acad J Sec Mil Med Univ,1999,20(9):   [点击复制]
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乙型肝炎病毒纯合子转基因小鼠(adr 1.2)的培育
胡以平,郝光荣,孙伟,王新民,李建秀,戴德顺,胡卫江
0
()
摘要:
目的:通过遗传学育种使外源基因整合位点随机的HBV转基因小鼠成为单一整合位点的纯合子转基因小鼠。方法:产生founder小鼠以后,将正常小鼠与转基因小鼠交配,通过PCR,Dot-blotting,Southern-blotting等方法检测 HBV DNA在转基因小鼠体内的整合状况,阳性率达50%左右后,进行近亲交配。结果:共得到了7代HBV转基因小鼠,其中包含有整合HBV基因纯合子的转基因小鼠。结论:外源基因在转基因小鼠体内可以稳定遗传,并得到了整合有HBV基因的纯合子转基因小鼠。 目的:通过遗传学育种使外源基因整合位点随机的HBV转基因小鼠成为单一整合位点的纯合子转基因小鼠。方法:产生founder小鼠以后,将正常小鼠与转基因小鼠交配,通过PCR,Dot-blotting,Southern-blotting等方法检测 HBV DNA在转基因小鼠体内的整合状况,阳性率达50%左右后,进行近亲交配。结果:共得到了7代HBV转基因小鼠,其中包含有整合HBV基因纯合子的转基因小鼠。结论:外源基因在转基因小鼠体内可以稳定遗传,并得到了整合有HBV基因的纯合子转基因小鼠。 目的:通过遗传学育种使外源基因整合位点随机的HBV转基因小鼠成为单一整合位点的纯合子转基因小鼠。方法:产生founder小鼠以后,将正常小鼠与转基因小鼠交配,通过PCR,Dot-blotting,Southern-blotting等方法检测 HBV DNA在转基因小鼠体内的整合状况,阳性率达50%左右后,进行近亲交配。结果:共得到了7代HBV转基因小鼠,其中包含有整合HBV基因纯合子的转基因小鼠。结论:外源基因在转基因小鼠体内可以稳定遗传,并得到了整合有HBV基因的纯合子转基因小鼠。
关键词:  肝炎病毒,乙型  小鼠,转基因  纯合子
DOI:
基金项目:
The generation of hepatitis B virus transgenic mice homozygote
胡以平,郝光荣,孙伟,王新民,李建秀,戴德顺,胡卫江
()
Abstract:
Objective: To obtain the homozygote of HBV transgenic mice with unique integrated site by genetic breeding. Methods: The positive transgenic mice were mated with the normal mice of the same line. And the target gene integration ratio were tested by PCR, Dot-blotting and Southern-blotting, and when the integration ratio was almost 50%, the positive mice were mated with inbreeding. Results: The 7 generations of HBV transgenic mice were obtained, and the homozygote of HBV transgenic mouse was obtained. Conclusion: The HBV DNA can be transmitted in the transgenic mice stably, and the homozygote of HBV transgenic mouse was obtained.
Key words:  hepatitis B virus  mice, transgenic  homozygote