Objective：To study the mechanism of neutral proteinases in degenerated cervical disc. Methods： Twenty white New Zealand rabbits were separated into 2 groups: model group and control group, each group consisted of 2 subgroup, each subgroup had 5 rabbits. The experimental model of degenerated cervical disc in the rabbit was established by resection of cervical supraspinous and interspinous ligaments and detachment of posterior paravertebral muscles from the cervical vertebrae. Rabbits in 3 month groups and in 8 month groups were killed 3 months and 8 months after operation respectively. The cervical disc tissues in all 4 groups were assayed to detect collagenase and serine proteinase activities by biochemical methods. Results： Collagenase and serine proteinase activities in discs from model subgroups were obviously higher than those of control subgroups. Collagenase and serine proteinase activities in discs from model subgroup in 3 month were higher than those of model subgroup in 8 month. Conclusion： Collagenase and serine proteinase activities in degenerated cervical discs are increased obviously, and their increase extents are not related to the extent of cervical disc degeneration. The results suggest that the collagenase and serine proteinase play an important role in early stage of the cervical disc degeneration.