Objective： To investigate TTV infection among blood donors with normal ALT, the transmission of TTV, and the association between TTV and hepatitis. Methods： Plasma samples from donors with normal ALT were detected for TTV DNA using PCR method, the PCR products (TTVD026) were sequenced directly. Sequence homology analysis was conducted by Internet BLAST. Results： TTV DNA was detected in 20 of 120 donors with normal ALT, the positive rate was 16.7%. Compared with TA278, GH1, TTVCHN1 and TTVCHN2, the nucleotide identity of TTVD026 were 99%, 99%, 99%and 89%, respectively. Conclusion： TTV infection exists in blood donors with normal ALT. TTVD026 might belong to the same genotype as TA278, GH1, TTVCHN1 and TTVCHN2, which indicate that blood transfusion may play an important role in the transmission of TTV.