Objective： To investigate the regulating role of Nei Yi Fang (NYF) on cytokines in endometriosis. Methods: According to Cummings AM method, animal models with endometriosis was established. IL-6 and IL-10 levels were determined by double antibody sandwich ELISA. Results: The peritoneal macrophage amounts and the production of IL-6 by LPS-activated peritoneal macrophages were remarkably higher in untreated group than that in the control group. The weights of endometriotic tissue and peritoneal macrophage amounts were remarkably lower in treated group compared with untreated group, and the levels of IL-6 by LPS-activated peritoneal macrophages were significantly reduced. The LPS-stimulated IL-10 secretion in peritoneal macrophages from untreated group was significantly lower compared with control group and treated group. Conclusion: The lost balance of IL-6 and IL-10 levels in endometriosis may be related to the pathogenesis of the disease. The treated mechanism of NYF was mediated by restraint of IL-6 composition, and promotion of IL-10 secretion.