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  • 凌昌全,潘瑞萍,李瑾,彭新萍,刘玉环,俞超芹.内异方对子宫内膜异位症IL-6、IL-10的调节作用[J].第二军医大学学报,1999,20(9):    [点击复制]
  • 凌昌全,潘瑞萍,李瑾,彭新萍,刘玉环,俞超芹.The regulating role of Nei Yi Fang on interleukin-6 and interleukin-10 in endometriosis[J].Acad J Sec Mil Med Univ,1999,20(9):   [点击复制]
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内异方对子宫内膜异位症IL-6、IL-10的调节作用
凌昌全,潘瑞萍,李瑾,彭新萍,刘玉环,俞超芹
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摘要:
目的:探讨中药内异方对子宫内膜异位症细胞因子IL-6、IL-10的调节作用。方法:根据Cummings AM方法建立子宫内膜异位症动物模型,采用ELISA法检测IL-6、IL-10含量。结果:非治疗组的腹腔巨噬细胞数量及巨噬细胞在脂多糖(LPS)刺激下产生的IL-6含量显著高于正常对照组;内异方治疗组异位种植内膜组织质量、腹腔巨噬细胞数量及巨噬细胞在LPS刺激下产生的IL-6水平显著低于非治疗组;非治疗组腹腔巨噬细胞在LPS刺激下产生的IL-10含量显著低于正常对照组和内异方组。结论:子宫内膜异位症细胞因子IL-6、IL-10的失衡与疾病的发生发展有关。内异方通过抑制IL-6的合成、促进IL-10的分泌,从而阻止了异位内膜组织的生长。
关键词:  子宫内膜异位症  内异方  白细胞介素6  白细胞介素10
DOI:
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The regulating role of Nei Yi Fang on interleukin-6 and interleukin-10 in endometriosis
凌昌全,潘瑞萍,李瑾,彭新萍,刘玉环,俞超芹
()
Abstract:
Objective: To investigate the regulating role of Nei Yi Fang (NYF) on cytokines in endometriosis. Methods: According to Cummings AM method, animal models with endometriosis was established. IL-6 and IL-10 levels were determined by double antibody sandwich ELISA. Results: The peritoneal macrophage amounts and the production of IL-6 by LPS-activated peritoneal macrophages were remarkably higher in untreated group than that in the control group. The weights of endometriotic tissue and peritoneal macrophage amounts were remarkably lower in treated group compared with untreated group, and the levels of IL-6 by LPS-activated peritoneal macrophages were significantly reduced. The LPS-stimulated IL-10 secretion in peritoneal macrophages from untreated group was significantly lower compared with control group and treated group. Conclusion: The lost balance of IL-6 and IL-10 levels in endometriosis may be related to the pathogenesis of the disease. The treated mechanism of NYF was mediated by restraint of IL-6 composition, and promotion of IL-10 secretion.
Key words:  endometriosis  Nei Yi Fang  interleukin-6  interleukin-10