Objective：To study the incidence, the antibiotics sensitivity and clinical characteristics of respiratory infection to H.influenzae in chronic obstructive pulmonary disease (COPD). Methods： Improved Columbia chocolate agar was used for the primary isolation of H.influenzae from sputums. The sensitivities of 20 antibiotics to H.influenzae were determined in vitro by Kirby-Bauer method and MICs of 12 antibiotics were determined by broth microdilution method. The clinical characteristics of respiratory infection to H.influenzae in COPD patients were analyzed. Results： Among 421 cases of COPD with acute acceleration, 229 strains of bacterium were isolated, 45(19.7%) were H.influenzae. The third generation of cephalosporins and the quinotones were most active against H.influenzae. The resistant rate against H.influenzae of Ampicillin was 22.2%.Conclusion：The infection due to H.influenzae is one of the most important agents leading to acute acceleration in COPD. The clinical characteristics of respiratory infection due to H.influenzae in COPD are no special.