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  • 刘苏,沈建伟,张兴荣,李石,邓志华,蔡洪培.As2O3诱导人胃癌细胞凋亡的研究[J].第二军医大学学报,1999,20(9):    [点击复制]
  • 刘苏,沈建伟,张兴荣,李石,邓志华,蔡洪培.As2O3 induces apoptosis of human gastric cancer cells[J].Acad J Sec Mil Med Univ,1999,20(9):   [点击复制]
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As2O3诱导人胃癌细胞凋亡的研究
刘苏,沈建伟,张兴荣,李石,邓志华,蔡洪培
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摘要:
目的:观察As2O3对人胃癌细胞 株 的作用,并探讨其抗胃癌作用的机制。方法: 不同浓度进口分析纯A s2O3 0.1-2.0 μmol/L与人胃癌细胞株共育一定时间后,观察细胞的 存活、形态学改变及细胞DNA含量的分布,并以NIH3T3细胞作对照。结果:1.0-2.0 μmol/L As2O3处理的胃癌细胞呈典型的凋亡特征性改变:在形态上 表现为胞膜完整、染色质固缩、核碎裂、凋亡小体形成;流式细胞术分析显示在G1期细胞 前出现亚二倍体峰,与As2O3作用的时间及浓度呈正相关趋势。结论:As2O3抗胃癌作用机制之一可能是诱导胃癌细胞凋亡。
关键词:  三氧化二砷  胃肿瘤  凋亡
DOI:
基金项目:
As2O3 induces apoptosis of human gastric cancer cells
刘苏,沈建伟,张兴荣,李石,邓志华,蔡洪培
()
Abstract:
Objective: To observe the mechanisms of imported highly purifi ed As2O3 inhibiting human gastric carcinoma cell lines. Methods: A wide range concentration of As2O3 (0.1-2.0 μmol/L) were cultured w ith gastric carcinoma cell lines SGC7901 and MKN45 with different time, the n, the number of active cells, cell morphology, cell-DNA content distribution were evaluated. NIH3T3 cells were as controls. Results: 1.0-2 .0 μm ol/L As2O3 treated cells presented some typical features of apoptosis such a s intact cell membrane, chromatin condensation, nucleic fragmentation and apopto tic body formation, The analysis of flow cytometery showed that sub-G1 cells immerged before the G1 phase, and show ed a positive correlation with the culture period and concentration of As2O3 . Agrose electrophoresis showed a specific DNA apoptotic ladder. Conclu sion: As2O3 can inhibit human gastric carcinoma cells, the induction of apoptosis is a very important mechanism of As2O3 to treat gastric carcinoma cells.
Key words:  As2O3  gastric neoplasms  apoptosis