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  • 胡云凤,吴月凤,陈思聪,张国元,秦永文,章同华,郑兴.心肌梗死大鼠梗死区和非梗死区间质胶原重构[J].第二军医大学学报,1999,20(9):    [点击复制]
  • 胡云凤,吴月凤,陈思聪,张国元,秦永文,章同华,郑兴.Collagen remodeling in infarct zone and non-infarct zone in a rat model of myocardial infarction[J].Acad J Sec Mil Med Univ,1999,20(9):   [点击复制]
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心肌梗死大鼠梗死区和非梗死区间质胶原重构
胡云凤,吴月凤,陈思聪,张国元,秦永文,章同华,郑兴
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摘要:
目的:研究心肌梗死(心梗)大鼠梗死区(IZ)和非梗死区(NIZ)间质胶原重构。方法:通过结扎左冠状动脉造成大鼠左心室大面积心梗。应用氯氨T法测定胶原含量,用SDS-PAGE分析胶原Ⅰ/Ⅲ型比值,动态观察大鼠心梗后1,3,7,14和42 d时IZ和NIZ胶原含量及胶原Ⅰ/Ⅲ比值的变化。结果: 3 d以后时间点IZ和NIZ胶原含量均明显大于假手术组相应时间的测值(P<0.05)。IZ胶原Ⅰ/Ⅲ比值3 d时降低,7 d以后明显升高(P<0.01)。NIZ在14 d时Ⅰ/Ⅲ比值明显升高。结论:在心梗后梗死区和非梗死区均发生间质胶原重构,这种重构在心梗后的左心室重构中可能发挥重要作用。
关键词:  心肌梗死  胶原
DOI:
基金项目:
Collagen remodeling in infarct zone and non-infarct zone in a rat model of myocardial infarction
胡云凤,吴月凤,陈思聪,张国元,秦永文,章同华,郑兴
()
Abstract:
Objective: To investigate collagen remodeling in infarct zone(IZ) and non-infarct zone(NIZ) in rat model of myocardial infarction. Methods:Large myocardial infarction of left ventricle was produced in rats by ligation of left coronary artery.Collagen contents were measured with the chloramine T method and collagen type Ⅰ/Ⅲ ratios were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.The changes of collagen content and collagen type Ⅰ/Ⅲ ratio in IZ and NIZ were sequentially observed at 1,3, 7,14, and 42 d after myocardial infarction. Results: Collagen contents in IZ and NIZ increased gradually with time and were higher at 3, 7, 14 and 42 d than those in sham group at corresponding time (P<0.05). Compared with sham group,collagen type Ⅰ/Ⅲ ratio in IZ was lower at 3 d, and increased significantly after 7 d(P<0.01). The collagen type Ⅰ/Ⅲ ratio in NIZ was higher at 14 d (P<0.01) and 42 d (P<0.05). Conclusion: Collagen remodelling occured in both infarct zone and non-infarct zone after myocardial infarction,which may play an important role in the process of left ventricular remodelling after myocardial infarction.
Key words:  myocardial infarction  collagen