发形霞水母毒素分离产物溶血活性的比较及其影响因素分析
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全军“十一五”专项资助项目(06Z020);上海市自然科学基金(06ZR14111);第二军医大学军事医学专项基金(05JS04).


Comparison of haemolytic activities of venom separations from jellyfish Cyanea capillata and their influencing factors
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Supported by the Foundation of the “Eleventh Five-Year Plan” for Medical Science Development of PLA (06Z020), Natural Science Foundation of Shanghai Municipal Government (06ZR14111) and Military Medical Foundation of Second Military Medical University(05JS04).

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    摘要:

    目的:分离发形霞水母刺丝囊毒素(nematocyst venom, NV)与无刺丝囊触手提取物(tentacle-only extract,TOE),比较二者的溶血活性,分析其影响因素。方法:采用自溶、离心方法分离发形霞水母NV和TOE,比较二者的溶血活性,观察提取物浓度、温度和pH值等因素对二者溶血活性的影响。结果:成功分离到NV与TOE;二者浓度相关溶血活性曲线均呈“S”形,溶血分数50%时所对应的质量浓度(HU50)分别为8 μg/ml 和67 μg/ml,前者溶血活性强度约为后者的8.4倍;温度对二者溶血活性影响较大,最大溶血活性都出现在40℃;pH值对二者溶血活性也有影响,在pH 8处二者都具有最大的溶血活性,但后者较前者更为敏感。结论:发形霞水母毒素分离产物NV和TOE均具有溶血活性,且前者强于后者;二者溶血活性受浓度、温度和pH值的影响。

    Abstract:

    Objective:To separate the nematocyst venom (NV) and tentacle-only extract (TOE) from the jellyfish Cyanea capillata and to analyze the difference between their haemolytic activities and the influencing factors.Methods: NV and TOE were separated by autolysis and centrifugation.The influence of concentration, temperature and pH value on the haemolytic activities of NV and TOE were observed.Results: NV and TOE were successfully separated.The concentration-associated haemolytic curves were “S” shaped for both NV and TOE.The HU50 of NV and TOE were 8 μg/ml and 67 μg/ml, respectively; and the haemolysis of NV was about 8.4 times of that of TOE.Temperature had great influence on haemolysis of both and the highest haemolysis were both at 40℃.pH value also had influence on haemolysis.The strongest haemolysis activity was found both at pH 8 and TOE was more sensitive.Conclusion: NV and TOE both have haemolysis activity,with the activity of NV stronger than that of the latter.The haemolysis activity is influenced by concentration, temperature and pH value.

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  • 收稿日期:2007-09-05
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  • 在线发布日期: 2008-01-21
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