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  • 聂菲1△,肖良1△,张静2,贺茜3,樊军文1,李玥1,张黎明1*.发形霞水母毒素分离产物溶血活性的比较及其影响因素分析[J].第二军医大学学报,2008,29(1):0083-0086    [点击复制]
  • NIE Fei1△, XIAO Liang1△, ZHANG Jing2, HE Qian3, FAN Jun-wen1, LI Yue1, ZHANG Li-ming1*.Comparison of haemolytic activities of venom separations from jellyfish Cyanea capillata and their influencing factors[J].Acad J Sec Mil Med Univ,2008,29(1):0083-0086   [点击复制]
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发形霞水母毒素分离产物溶血活性的比较及其影响因素分析
聂菲1△,肖良1△,张静2,贺茜3,樊军文1,李玥1,张黎明1*
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(1.第二军医大学海军医学系防化医学教研室,上海 200433; 2.第二军医大学药学院药理学教研室,上海 200433; 3.第二军医大学长海医院妇产科,上海 200433)
摘要:
目的:分离发形霞水母刺丝囊毒素(nematocyst venom, NV)与无刺丝囊触手提取物(tentacle-only extract,TOE),比较二者的溶血活性,分析其影响因素。方法:采用自溶、离心方法分离发形霞水母NV和TOE,比较二者的溶血活性,观察提取物浓度、温度和pH值等因素对二者溶血活性的影响。结果:成功分离到NV与TOE;二者浓度相关溶血活性曲线均呈“S”形,溶血分数50%时所对应的质量浓度(HU50)分别为8 μg/ml 和67 μg/ml,前者溶血活性强度约为后者的8.4倍;温度对二者溶血活性影响较大,最大溶血活性都出现在40℃;pH值对二者溶血活性也有影响,在pH 8处二者都具有最大的溶血活性,但后者较前者更为敏感。结论:发形霞水母毒素分离产物NV和TOE均具有溶血活性,且前者强于后者;二者溶血活性受浓度、温度和pH值的影响。
关键词:  发形霞水母;刺丝囊毒素;无刺丝囊触手提取物  溶血活性
DOI:10.3724/SP.J.1008.2008.00083
投稿时间:2007-09-05
基金项目:全军“十一五”专项资助项目(06Z020);上海市自然科学基金(06ZR14111);第二军医大学军事医学专项基金(05JS04).
Comparison of haemolytic activities of venom separations from jellyfish Cyanea capillata and their influencing factors
NIE Fei1△, XIAO Liang1△, ZHANG Jing2, HE Qian3, FAN Jun-wen1, LI Yue1, ZHANG Li-ming1*
(1.Department of Chemical Defense Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China; 2.Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433; 3.Department of Gynecology and Obstetrics, Changhai Hospital, Second Military Medical University, Shanghai 200433)
Abstract:
Objective:To separate the nematocyst venom (NV) and tentacle-only extract (TOE) from the jellyfish Cyanea capillata and to analyze the difference between their haemolytic activities and the influencing factors.Methods: NV and TOE were separated by autolysis and centrifugation.The influence of concentration, temperature and pH value on the haemolytic activities of NV and TOE were observed.Results: NV and TOE were successfully separated.The concentration-associated haemolytic curves were “S” shaped for both NV and TOE.The HU50 of NV and TOE were 8 μg/ml and 67 μg/ml, respectively; and the haemolysis of NV was about 8.4 times of that of TOE.Temperature had great influence on haemolysis of both and the highest haemolysis were both at 40℃.pH value also had influence on haemolysis.The strongest haemolysis activity was found both at pH 8 and TOE was more sensitive.Conclusion: NV and TOE both have haemolysis activity,with the activity of NV stronger than that of the latter.The haemolysis activity is influenced by concentration, temperature and pH value.
Key words:  Cyanea capillata  nematocyst venom  tentacle-only extract of jellyfish  haemolysis activity