| 摘要: |
| 目的：分离发形霞水母刺丝囊毒素（nematocyst venom, NV）与无刺丝囊触手提取物（tentacle-only extract,TOE），比较二者的溶血活性，分析其影响因素。方法：采用自溶、离心方法分离发形霞水母NV和TOE，比较二者的溶血活性，观察提取物浓度、温度和pH值等因素对二者溶血活性的影响。结果：成功分离到NV与TOE；二者浓度相关溶血活性曲线均呈“S”形，溶血分数50％时所对应的质量浓度（HU50）分别为8 μg/ml 和67 μg/ml，前者溶血活性强度约为后者的8.4倍；温度对二者溶血活性影响较大，最大溶血活性都出现在40℃；pH值对二者溶血活性也有影响，在pH 8处二者都具有最大的溶血活性，但后者较前者更为敏感。结论：发形霞水母毒素分离产物NV和TOE均具有溶血活性，且前者强于后者；二者溶血活性受浓度、温度和pH值的影响。 |
| 关键词: 发形霞水母；刺丝囊毒素；无刺丝囊触手提取物 溶血活性 |
| DOI：10.3724/SP.J.1008.2008.00083 |
| 投稿时间：2007-09-05 |
| 基金项目:全军“十一五”专项资助项目(06Z020);上海市自然科学基金(06ZR14111);第二军医大学军事医学专项基金（05JS04）. |
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| Comparison of haemolytic activities of venom separations from jellyfish Cyanea capillata and their influencing factors |
| NIE Fei1△, XIAO Liang1△, ZHANG Jing2, HE Qian3, FAN Jun-wen1, LI Yue1, ZHANG Li-ming1* |
| (1.Department of Chemical Defense Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China; 2.Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433; 3.Department of Gynecology and Obstetrics, Changhai Hospital, Second Military Medical University, Shanghai 200433) |
| Abstract: |
| Objective：To separate the nematocyst venom (NV) and tentacle-only extract (TOE) from the jellyfish Cyanea capillata and to analyze the difference between their haemolytic activities and the influencing factors.Methods: NV and TOE were separated by autolysis and centrifugation.The influence of concentration, temperature and pH value on the haemolytic activities of NV and TOE were observed.Results: NV and TOE were successfully separated.The concentration-associated haemolytic curves were “S” shaped for both NV and TOE.The HU50 of NV and TOE were 8 μg/ml and 67 μg/ml, respectively; and the haemolysis of NV was about 8.4 times of that of TOE.Temperature had great influence on haemolysis of both and the highest haemolysis were both at 40℃.pH value also had influence on haemolysis.The strongest haemolysis activity was found both at pH 8 and TOE was more sensitive.Conclusion: NV and TOE both have haemolysis activity,with the activity of NV stronger than that of the latter.The haemolysis activity is influenced by concentration, temperature and pH value. |
| Key words: Cyanea capillata nematocyst venom tentacle-only extract of jellyfish haemolysis activity |
