In vitro evaluation of biocompatibility and surface modification of 3 kinds of artificial graft material
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    Abstract:

    Objective:To evaluate the biocompatibility of 3 kinds of artificial,biodegradable graft materials, including T20 [Poly ( butylene terephthalate)-co-poly ( cyclohexylene dimethanol terephthalate)-b-poly ( ethylene glycol)], PHBV ( Poly3hydroxybutyrate-Poly3-hydroxyvalerate) and PEGT/PBT [Poly (butylene terephthalate)/poly (ethylene glycol) terephthalate], and to study their effects on canine smooth muscle cells(SMCs) growth after surface modification. Methods: The contact angles were measured to compare the hydrophilicity of T20, PHBV, and PEGT/PBT scaffolds. Then SMCs were cultivated on these biodegradable scaffolds; their proliferation and growth were assayed by MTT assay, FITC and electron microscopic observation for evaluation of their in vitro biocompatibility. The growth of SMCs was also assayed on the 3 scaffolds after they were pretreated with gelatin or Poly-l-lysine. Results: The contact angles of 3 scaffolds were all less than 90°(PEGT/PBT〉T20〉PHBV). MTT assay showed that SMCs adhered to and grew well on T20, PHBV, and PEGT/PBT scaffolds. The cell viability began to increase 24 h after cultivation (PEGT/PBT〉T20〉PHBV, P〈0.01), followed by a decrease, but increased again 72 h after cultivation (T20〉PEGT/PBT〉PHBV, P〈0.01). FITC observation showed that SMCs grew well and electron microscopic observation showed a confluent growth of SMCs with abundant extracellular matrix. The viability of SMCs was significantly higher on scaffolds pretreated with gelatin or Poly-l-lysine than on those without pretreatment(P〈0.01). Conclusion: T20,PHBV,PEGT/PBT have good in vitro biocompatibility; the scaffolds made of these materials can obviously improve cell attachment after surface modification.

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