Objective：To investigate the resources of medicinal microorganisms of the East China Sea. Methods： The sea water and sediment samples were collected from the East China Sea. Different culture media were used to isolate the microorganism strains by infinite dilution and streak plate procedure. Then the isolated strains were screened for antimicrobial active strains using paper disc diffusion model and for antimitosis active strains using Pyricularia Oryzae model. Results： A total of 1 041 strains of marine microorganisms were isolated from marine water and sediment samples, including 506 actinomycetes, 114 fungi and 421 bacteria. It was found that 303（29.1 %） strains of marine microorganisms had antimicrobial activity and 219 （21.0%） strains had antimitotic activity. Conclusion.. The proportions of strains with antimicrobial activities and antimitotic activities are both above 20%, suggesting that the East China Sea has rich resources of medicinal microorganisms.