Objective： To investigate the anti-myeloma cells specific immune activity induced by myeloma protein-pusled dendritic cells（DCs）. Methods： DCs were obtained from peripheral blood（PB） of multiple myeloma（MM） patients after co-culture with GM-CSF,IL-4 and TNF-α. The phenotype of DCs was examined by flow cytometry. Host lymphocytes were stimulated with DCs pulsed with myeloma protein acquired from serum of MM patients on DEAE-celluose; lymphocytes treated with unpulsed-DC served as control. [^3 H]-thymidine incorporation and 4 h ^51 Cr-release assay were used to detect the proliferation and cytotoxicity against different targets（auto-myeloma cells,U266 cells） by T lymphocytes. Results： In vitro proliferation and cytotoxicity of T lymphocytes activated by myeloma protein pulsed-DCs were greater than those of T lymphocytes in the control group（P〈0.01）. T lymphocytes in neither of the 2 groups had notable cytotoxicity against U266 cells. Conclusion： DCs pulsed by myeloma protein can effectively induce specific cytotoxicity of T lymphocytes against myeloma cells