Objective：To develop a compound staining method showing collagenic, elastic fibers and melanocyte in skin tissues. Methods： A staining method combining Masson Fontana silver nitrate, Ponceau S, Picric acid and Victoria blue （MFSNP-PA-VB） was used in the present animal skin experimental. Results： The melanin granule was black in the skin melanocyte, collagenic fibers were red, the red blood cells at hemorrhagic focus were yellow, and the elastic fibers were green by this MFSNP-PA-VB staining method. Conclusion： This compound staining method has multicolor and fine contrast, and can better show melanins, the red blood cells at hemorrhagic focus, and collagenic and elastic fibers