Objective： To investigate whether there is pharmacokinetic interaction between amlodipine and irbesarta when they are administered together into beagle dogs. Methods： Six healthy adult beagle dogs were evenly divided into 3 groups according to a self-controlled randomized 2-way crossover design. Animals in 3 groups were given a single oral dose of 5 mg amlodipine powder, 250 mg irbesartan powder and compound powder of amlodipine and irbesartan （containing 5 mg amlodipine and 250 mg irbesartan）, respectively. The concentrations of amlodipine and irbesartan in beagle dog plasma were determined at specified time points by HPLC-ESI-MS/MS and HPLC-Fluorescence method, respectively; the pharmacokinetic parameters were calculated subsequently. Results： Cm,xand AUC0-∞ of amlodipine were （267.63±38.49） ng/ml and （5 648.71±871.68） ng · h/ml when administered alone and were （267.43±37.97） ng/ml and （5 751.60±924.49） ng · h/ml when combined with irbesartan,respectively. Cmaxand AUC0-∞ of irbesartan were （2 623.56±198.65） ng/ml and （10 327.53±1 522.58） ng · h/ml when administered alone and were （2 345.61±42.32） ng/ml and （10 217.12±1 239.13） ng · h/ml when combined with amlodipine,respectively. There was no significant difference in cmax and AUC of the 2 drugs when they were used alone or jointly. Conclusion： There is no obvious pharmacokinetic interaction between amlodipine and irbesarta when they are administered jointly into beagle dogs.