Objective：To investigate the bioactive chemical constituents in Bidens bipinnata L. for treatment of diabetes. Methods： Extraction was done with 80% EtOH; isolation and purification were carried out on silica gel,C18 silica gel column, and Sephadex-LH20 column; and the chemical structures of the products were identified by physicochemical properties and spectral analysis. Results： Nine compounds were obtained from Bidens bipinnata L. and their chemical structures were identified as： quercitin（ Ⅰ ）, hyperoside （ Ⅱ ） ,quercitin-7-O-rhamnopyranoside（ Ⅲ ） ,6,7,3 ＇4 ＇-tetrahydroxy aurone（ Ⅳ ） ,4,5-di-O- caffeoyl quinic acid（ Ⅴ ） ,stigmasterol 3-O-glucopyranoside （ Ⅵ ）, ethyl 3,4-dihydroxybenzoate（ Ⅶ ）, okanin（ Ⅷ ）, and luteolin（ Ⅸ ）. Conelusion： Except for okanin and hyperoside,the rest 7 compounds are isolated from the plant for the first time.