Objective：To establish a mouse model of human orbital rhabdomyosarcoma（RD）. Methods： RD cells were cultured and implanted subcutaneously into the costal regions of nude mice and severe combined immunodeficiency （SCID） mice （4 weeks old）. The nude mice were radiated by ^60Co 1 day before the injection. The rhbdomyosarcoma tissue masses were harvested from nude mice and were subcutaneously implanted into the costal regions of another group of BALB/c mice （tumor tissue mass implantation）. The tumor growth was observed in all groups and the growth curves were ploted. H-E staining was used to examine pathology of the tumor tissues. Results： Solid tumors were palpable 6-8 weeks after implantation in ^60Co radiated nude mice and about 3-4 weeks after implantation in SCID mice. The solid tumors were obviously seen in the nude mice 2 weeks after tumor tissue mass transplantation. The tumor forming rates were 100% in all groups. The tumor growing curves were successfully ploted and the tumors had a progressive growth. Pathological findings of the tumor tissue were similar to those of human orbital rhabdomyosarcoma. Conclusion： The human orbital rhabdomyosarcoma models have been successfully established in nude mice and SCID mice. Tumor tissue mass implantation can shorten the tumor forming time in BALB/c nude mice. This research lays a foundation for further study or human orbital rhabdomyosarcoma.