Protective effect of activated AMP-activated protein kinase on palmatic acid-induced damage of human umbilical vein endothelial cells
CSTR:
Author:
Affiliation:

Clc Number:

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    Objective:To investigate the protective effect of activated AMP-activated protein kinase (AMPK) on the human umbilical vein endothelial cell(HUVEC) damage induced by palmatlc acid (PA) and the related mechanism. Methods: HUVEC were divided into 8 groups according to the culture media (cultured for 24 to 72 h): blank control (conventional culture), 300 μmol/L PA, 1 mmol/L aminoimidazole-4-carboxamide-l-beta-ribofuranoside (AICAR), PA+ AICAR, 2 mmol/L metformin, PAq-metformin, 10 btmol/L pioglitazone, and PAq-pioglitazone groups. Survival rates of HUVEC were determined by MTT at 24, 48 and 72 h after culture. Western blot were performed to detect the expression of phospha-AMPK at 24 h after culture. Results: Compared with blank control group, cell survival rates in PA group were decreased by 58.95M, 36.68% and 15.09M at 24, 48 and 72 h(P〈0.05), respectively. Seventy-two hours after culture, HUVEC survival rates of AICAR-t-PA group, Metnt-pA group and PGZ+PA group were obviously higher than that of PA group (P〈0.05) ; AICAR, metformin or pioglita- zone alone had no obvious influence on HUVEC survival rate. Twenty-four hours after culture, Phospha-AMPK expression was increased in AICAR, metforrnin and pioglitazone groups compared with that of control group (P〈0.05). Conclusion: AICAR, metformin and pioglitazone can decrease the damage of HUVEC induced by PA, possibly through activating AMPK

    Reference
    Related
    Cited by
Related Videos

Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:January 09,2006
  • Revised:July 15,2006
  • Adopted:
  • Online: September 20,2006
  • Published:
Article QR Code