Objective： To modify the pulsatile bioreactor we constructed previously for simulating the high-flow, highpressure hemodynamics of heart valve in vivo, and to experimentally cultivate the tissue-engineered heart valves （TEHV） in the modified bioreactor. Methods： T-PLS system （NewheartBio Co. ,Ltd Korea） was used to generate pulsatile flow in the modified bioreactor and we designed a new air-exchange pathway to avoid contamination. The TEHV were made by seeding human bone mesenchymal stem cells （BMSCs） on deellularized porcine heart valve. After cultured under static condition for 4 d, the TEHVs were moved to the modified bioreactor and exposed to low-flow （0-600 ml/min） or high-flow（0-4 800 ml/min） pulsatile hydrodynamics for 7d, then the cells on TEHVs were evaluated. Results： After modification, the flow range expanded from （0- 1 200） ml/min to （0-6 000） ml/min and the pressure range expanded from （0-40） mmHg to （0-180） mmHg. In culture experiments, 26.3 % of the seeded cells remained under low-flow environment and cells were completely lost under the high-flow dynamics. Conclusion： The modified bioreactor can basically simulate the dynamics of heart valve in vivo and can be used in TEHV cultivation research. However, the current TEHV can not tolerate the high-flow pulsatile hydrodynamics