Objective：To search for a new method for synthesis of 9-fluorenylmethoxycarbonyl amino acid tert-butyl ester. Methods： Glycine, L-Proline, and L-Phenylanaline were separately allowed to react with 9-fluorenylmethylchloroformate to obtain the corresponding 9-fluorenylmethoxycarbonyl amino acids. With 4-（dimethylamine） pyridine （DMAP） used as catalyst, the 9-fluorenylmethoxycarbonyl amino acids were allowed to react with Tert-butyl dicarbonate for the corresponding 9-fluorenyl- methoxycarbonyl amino acid tert-butyl esters. Results： Three 9-fluorenylmethoxycarbonyl amino acid tert-butyl esters were successfully synthesized by this method and their structures were confirmed by 1^HNMR. Conclusion： Ours is a simple method with mild condition and high yielding rate for synthesis of 9-fluorenylmethoxycarbonyl amino acid tert-butyl esters.