Objective：To make a comparative study on the root and stalk of Actinidia valvata, so as to lay a ground for expanding medicinal part of Actinidia valvata. Methods: A comparative study was made between the root and stalk of Actinidia valvata on the following aspects: macroscopical properties, physical and chemical properties, contents of active constituents, and pharmacological actions. Results: The root and stalk of Actinidia valvata both showed positive reaction for alkaloids and saponin, and both had 280 nm absorption peak in UV-Vis spectrum. The aqueous extraction yield of the root and stalk were 5.35% and 5.68%, respectively; the contents of dihydrodehydrodiconiferyl alcohol were 0.005 01% and 0.006 41%, respectively. The IC50 of root and stalk extracts against HL60 were 62.39 mg and 75.51 mg, respectively; and against K562 were 70.47 mg and 77.46 mg, respectively. Their extracts had similar scavenging activity for free radicals and reductive effect; neither of them had analgesia actions.Conclusion: The root and stalk of Actinidia valvata have similar physical and chemical properties, contents of active constituents and pharmacological actions, suggesting that it is highly possible to substitute the root of Actinidia valvata with stalk for medicinal usage.