Objective：To separate the nematocyst venom (NV) and tentacle-only extract (TOE) from the jellyfish Cyanea capillata and to analyze the difference between their haemolytic activities and the influencing factors.Methods: NV and TOE were separated by autolysis and centrifugation.The influence of concentration, temperature and pH value on the haemolytic activities of NV and TOE were observed.Results: NV and TOE were successfully separated.The concentration-associated haemolytic curves were “S” shaped for both NV and TOE.The HU50 of NV and TOE were 8 μg/ml and 67 μg/ml, respectively; and the haemolysis of NV was about 8.4 times of that of TOE.Temperature had great influence on haemolysis of both and the highest haemolysis were both at 40℃.pH value also had influence on haemolysis.The strongest haemolysis activity was found both at pH 8 and TOE was more sensitive.Conclusion: NV and TOE both have haemolysis activity,with the activity of NV stronger than that of the latter.The haemolysis activity is influenced by concentration, temperature and pH value.