Objective：To establish a method by combining microwave-assisted extraction(MAE), solid phase extraction(SPE) and high performance liquid chromatography (HPLC) for determination of the mefenacet residues in rice.Methods: Acetone and acetontrile (37) were used as extraction solvent. Microwave-assisted extraction was used to extract mefenacet residues in the rice. The extracts were then cleaned up with a Florisil cartridge and then subjected to Hypersil C18 column（5 μm，4.6 mm×200 mm ）, with acetonitrilewater（5050，V/V）solution as mobile phase and with a flow rate of 1.0 ml/min; the ultraviolet detection wavelength was at 217 nm. Results: Good linear correlation for mefenacet was found within a concentration range of 0.198-9.900 μg/ml. The detection limit was 0.039 6 μg/mL for mefenacet（S/N=2）. The average recovery rate of rice hull and brown rice were 90.8% (RSD 1.8%) and 85.6% (RSD 2.5%)，respectively.Conclusion: The present method is simple and rapid; it can be used for the determination of mefenacet residues in rice.