Objective:To investigate the chemical constituents in the EtOAc fraction of the EtOH extract of Duchesnea indica Andr.Focke.Methods: Extraction was done with 95% EtOH.EtOAc fraction of the EtOH extract was isolated and purified by column chromatography using silica gel,RP-C18,Sephadex LH-20 and HPLC.The structures of the compounds were identified by their physico-chemical properties and spectral analysis.Results: Thirteen compounds were obtained from the EtOAc fraction of Duchesnea indica Andr.Focke and they were identified as euscaphic acid(1),arjunic acid(2),p-hydroxy cinnamic acid(3),apigenin(4),kaempferol(5),2α-hydroxy ursolic acid(6),2α-hydroxy oleanolic acid(7),tomentic acid(8),kaii-ichigeside F1(9),potengriffioside A(10),rosamultin(11),kaempferol-3-O-β-D-glucoside(12),and isoquercitrin(13).Conclusion: The compounds 2-5,10,and 12 have been isolated from the genus of Duchesnea for the first time.