Objective:To prepare and characterize nobilisideA freeze-dried liposome. Methods: The nobilisideA liposomes were prepared using a film dispersion method followed by a freeze-dried technique. The freeze-dried liposomes were characterized with regard to the drug content, entrapment efficiency, the particle size, ζ potential, and its stability and hemolytic rate in glucose transfusion. Results: Through optimized technique, the prepared nobilisideA freeze-dried liposome, with sucrose as protective agent, had satisfactory structure and redissolubility. The contents of nobilisideA liposome before and after freeze-drying were 108.3% and 108.6%, the entrapment efficiency were 98.4% and 97.8%, the particle sizes were 89.9 nm and 115.8nm, and the ζ potentials were -19.9mV and -15.9mV, respectively. The liposome content, entrapment efficiency, particle size and hemolytic rate were stable within 8 h during glucose transfusion. Conclusion: NobilisideA freeze-dried liposomes have a narrow size distribution range, a high entrapment efficiency and a stable character, making it a promising candidate for nobilisidesA liposome.