Objective:To investigate the anti-inflammatory and analgesic activity of Eucommiae ulmoides seed total glycoside(EUG). Methods: Dimethyl benzene(DB)-induced mouse ear edema model and carrageein-induced rat paw edema model were employed for the anti-inflammatory activity experiment. The experiment for analgesic activity was done using hot plate test, photoelectricity-induced test and acetic acid-induced writhing method. Results: EUG at 110,220 mg/kg showed an obvious inhibitory effect against DB-induced mouse ear edema volume, and at 55， 110, 220 mg/kg it showed an obvious analgesic effect against carrageein-induced rat paw edema rate 4 hours after oral administration. At 220 mg/kg EUG notably raised the pain threshold in the mice stimulated by hot plate and photoelectricity. At 110,220 mg/kg EUG greatly reduced the number of acid-induced writhing. Conclusion: The result suggests that EUG has potential anti-inflammatory and analgesic activity.