Objective:To develop a new method for the simultaneous determination of two hydrophilic components and two lipophilic components of Radix et Rhizoma Salviae Miltiorrhizae.Methods: The HPLC-DAD method was employed using a column of Agilent Zorbax TC C18 (4.6 mm×250 mm, 5 μm) with a mobile phase of methanol -2% acetic acid.The gradient elution program was as follow:0-15 min, 30% B-40% B; 15-20 min, 40% B-60% B; 20-25 min, 60% B-90% B;25-40 min, 90% B.The detection wavelength was set at 281 nm and the temperature was 35℃.Results: The linearity was obtained over 3.76-120.20 μg·ml-1(r=0.999 9) for rosmarinic acid, 34.20-109 4.5 μg·ml-1(r=0.999 9) for salviamolic acid B, 0.64-20.32 μg·ml-1(r=0.999 9) for clyptotanshinon,and 1.02-32.72 μg·ml-1(r=0.999 6) for tanshinone ⅡA.The RSDs of precision and stability of the sample were both less than 1% in 48 hours.The average recovery was between 99.72%-100.63%.Conclusion: The present method is simple and has satisfactory efficacy; it can simultaneously determine multiple hydrophilic and lipophilic bioactive components in Salvia miltiorrhiza from different areas.