Objective:To determine meloxicam concentrations in both plasma and extract solution of tested-skin in SD rats and the fluorescence intensity,location of fluorescein sodium salt (NaFI) in striped rat skin within 4 h after transdermal administration,so as to investigate the enhancing effect of negative electret on percutaneous absorption of meloxicam and percutaneous absorption route of NaFI.Methods: Pharmaceutical method and grid-controlled constant corona charge technique were used to prepare electret meloxicam patch.High performance liquid chromatography (HPLC) method was employed to determine meloxicam concentration after transdermal administration.NaFI was used as probe to determine the localization and percutaneous absorption route of NaFI by using laser scanning confocal microscopy (LSCM).Results: (1)Negative electret and its polypropylene(PP) electret meloxicam patch exhibited a good charge storage stability.(2)The results of HPLC demonstrated that electret had a remarkable enhancing effect on percutaneous absorption of meloxicam after application for 1-4 h.(3)LSCM further proved the enhancing effect of negative electret on percutaneous absorption of small molecules.We also found that the stratum corneum and the hair follicle areas were the two main pathways for the enhancing effect of the electret.Conclusion: Negative electret can be used as an enhancer for transdermal permeation of meloxicam.