Objective: To design a portable real-time monitoring tool for spine surgery and to preliminarily evaluate its application, so as to reduce the iatrogenic injury during the treatment of battle field spinal cord injury. Methods: A portable spine guiding device for spinal fixation was designed and prepared. The spinal specimens were randomly divided into control group (n=10) and experimental group (n=10). In the control group the way was cleared for the pedicle according to the doctor's experience; in the experimental group the way was cleared using our self-designed guiding device. The operation time was recorded in the two groups. After manipulation the specimens were opened along the pedicle level; the location and the depth of the needle were compared. Results: The manipulation time of a single vertebral body was (0.5±0.2) min in the control group and (0.6±0.1) min in the experimental group (P>0.05). The edge of the puncture to the pedicle wall in the control group was significantly shorter than that in the experimental group([1.1±0.3] vs [1.8±0.2]mm,P=0.037).Conclusion:The designed portable guiding device for spinal fixation is easy to carry, simple to operate; and initial in vitro application is satisfactory and can provide reliable reference for early operation.