Objective:To design and prepare HBc virus-like particle vaccine (VLP) harboring the specified epitope of gastric inhibitory peptide (GIP),and to study its immune effects,so as to cast new lights on immune therapy of obesity.Methods: GIP cDNA was cloned and was fused with HBc VLP (1-144aa) cDNA.Through prokaryotic expression and purification processes,a GIP vaccine linked to the HBc-derived VLP carrier was constructed.We also cloned the fusion gene into eukaryotic vector pVAX1 to prepare GIP nuclear acid vaccine.And the two kinds of vaccines were tested in rats for their immunological parameters.Results: GIP vaccine linked to HBc-derived VLP carrier was successfully prepared.We also prepared the genetic vaccine pVAX1-HBc-GIP.The combined vaccination with both vaccines could obtain high titer and peptide-specific IgG antibody,displaying a satisfactory immunogenicity.Conclusion: GIP protein vaccine using HBc as immune-enhancing vaccine carrier can effectively induce GIP-specific humoral immunity,disrupting the tolerance to self antigens in rats.The vaccine is worth studying as a new drug for control of obesity.