Objective:To investigate the expression of PIAS2 in the testis tissues of patients with different spermatogenic functions.Methods:Eighty testicular biopsy samples from patients with azoospermia were subjected to pathological diagnosis and were divided into the following groups according to the pathological findings:normal or basically normal spermatogenesis group (n=40),hypo-spermatogenesis group (HS,n=20),and sertoli cell-only syndrome group (SCOS,n=20).Immunohistochemical method (SP) was used to examine the expression of PIAS2 in the testis tissues with different spermatogenic functions.Results:PIAS2 was strongly positive (dark brown) in the spermatogenic cells of different functions.It was weakly positive or absent in the Leydig cells and Sertoli cells.Conclusion:The status of PIAS2 in the testicular tissues is associated with the spermatogenic functions,and it may serve as a potential parameter for predicting of spermatogenic function.