Abstract:Objective:To investigate the role of SDF1/CXCR4 in metastasis of renal cell carcinoma and to observe the intracellular location of different CXCR4 segments in renal carcinoma cells.Methods: The potential nuclear localization sequences of different CXCR4 were discovered by nuclear localization software and experiments.Full length and truncated forms of CXCR4 were fused with green fluorescent protein pEGFPN1 and their influence on subcellular localization was examined by confocal microscopy after transfecting them into renal carcinoma cell line A498.Results: Analysis with PSORT Ⅱ Prediction revealed that the nuclear localization sequence region of CXCR4 was located between amino acids 146 and 149(RPRK).Expression products of the recombinant plasmids with SDF1 stimulation,including EGFPCXCR4(1510 bp),EGFPCXCR4(1765 bp) and wildtype EGFPCXCR4,were mainly located in the cell nuclei.However,expression product of EGFPCXCR4(1267 bp) with SDF1 stimulation was mainly located in the renal cell cytoplasm.Expression product of wildtype EGFPCXCR4 full length plasmid without SDF1 stimulation was mainly located in the cell cytoplasm; these results accorded with the results of bioinformatics analysis.Conclusion: Nuclear localization sequence of CXCR4 is located in the amino acids 90 to 170,which provides a theoretical basis for further clarifying the nuclear localization sequences of CXCR4 in renal cell carcinoma cells and for finding new potential target for inhibiting the metastasis of renal cell carcinoma.