Objective To study the antifungal activities of triterpene glycosides derived from the sea cucumber Actinopyga sp. Methods Triterpene glycosides were isolated and purified by silica gel chromatography, reversed-phase silica gel chromatography, Sephadex LH-20, and RP-HPLC from Actinopyga sp. The structures of the triterpene glycosides were elucidated based on spectral data, chemical reactions, and related references. The antifungal activities of the extracts against three strains:Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus were assessed by liquid microdilution method. Results Four triterpene glycosides were identified from Actinopyga sp.; and they included frondoside A (1),pervicoside C (2), holothurin A (3), and holothurin B (4). The MIC80 of compound 2 and compound 3 for antifungal effect were 1-4 mg/L, showing marked antifungal activities.Conclusion Compounds 1 and 2 have been isolated from this halobios for the first time. All the four compounds show considerable antifungal activities, and pervicoside C may be used as a lead compound of antifungal agents.