ObjectiveTo study the electrochemical behavior of bupivacaine hydrochloride and to establish an electrochemical method for determination of bupivacaine hydrochloride.MethodsThe electrochemical behavior of bupivacaine hydrochloride was studied by cyclic voltammetry (CV), and differential pulse voltammetry (DPV) was used to determine the contents of bupivacaine hydrochloride.ResultsIn a 0.01 mol/L tetrabutylammonium perchlorate (TBAP) buffer solution, the oxidization peak current on the platinum electrode in the bupivacaine hydrochloride solution was in a good linear relation over a concentration range of 1.0×10-5-2.0×10-4 mol/L, with the r value being 0.995 8, and a detection limit of 1×10-5 mol/L (by DPV).ConclusionOur method is accurate, easy to perform, and with good repeatability, and it can be used to determine bupivacaine hydrochloride content in bupivacaine hydrochloride raw medicine.