Objective To examine the relationship between G72 gene locus (an important gene of NMDA receptor pathway) and schizophrenia in Chinese females. Methods We genotyped three single-nucleotide polymorphisms(rs3916965,rs3916967 and rs2391191) in G72 gene locus region in 545 Chinese females of Han ethnicity(260 schizophrenia cases and 285 healthy controls)and performed the linkage disequilibrium analysis. Results Significant association was found between two SNPs and schizophrenia (P=0.038,6 for the allele G of rs3916967 and P=0.009,6 for the allele A of rs2391191). Furthermore, three SNPs were used in multiple haplotype analysis and significant difference was found for the common haplotype GAG(P=0.002,4). Conclusion G72 gene may be a candidate susceptible gene for schizophrenia in the Chinese females of Han ethnicity. Further efforts are warranted to confirm our findings.