[Abstract]ObjectiveTo study the influence of Salvia miltiorrhiza bge.f.alba(SMA) on apoptosis and Bcl-2 expression in human umbilical vein endothelial cell (HUVEC) in vitro.MethodsHUVECs were isolated using perfusion and enzyme digestion methods, and the obtained cells were identified by morphological observation and ⅧAg immunoreactivity examination. The cells in the exponential phase of growth were treated with H2O2 and different concentrations of SMA(high dose: 0.10 g/ml, low dose: 0.01 g/ml). The cell apoptosis was determined by flow cytometric analysis and Bcl-2 expression was examined by immunofluorescence mehtod. MethodsSMA significantly decreased the H2O2-induced apoptosis of HUVECs (P<0.01 in high dose group and P<0.05 in low dose group), and significantly increased the expression of Bcl-2 in high dose group(P<0.01). MethodsSMA can inhibit H2O2-induced apoptosis of HUVEC, which might be associated with the increase of Bcl-2 expression.