ObjectiveTo investigate the chemical constituents of 80% ETOH extract from rhizome of Belamcanda chinensis (L.) DC. MethodsThe constituents were isolated and purified by chromatography on silica gel, Sephadex LH-20 and HPLC; the structures of the compounds were determined by MS and NMR spectral analysis. ResultsTen constituents were identified from rhizome of Belamcanda chinensis (L.) DC, including tectoridin (Ⅰ), acetovanillone (Ⅱ), 4-hydroxy-acetophenone (Ⅲ), β-sitosterol (Ⅳ), β-daucosterol(Ⅴ), 5,7,4′-trihydroxyl-3′,5′-dimethoxyflavone (Ⅵ), luteolin (Ⅶ), apigenin (Ⅷ), 5,7,4′-trihydroxyflavanones (Ⅸ), and isorhamnetin (Ⅹ).ConclusionCompounds Ⅵ, Ⅶ, Ⅷ, and Ⅸ have been isolated from rhizome of Belamcanda chinensis for the first time.