[Abstract]ObjectiveTo establish a multivariate reference range for ECG while considering the relation between multiple variables. MethodsA total of 777 female undergraduates in a university were taken as the subjects and the P-wave amplitude was taken as an example to establish a multivariate reference range for P-wave amplitude by using Mahalanobis Depth Function. MethodsP-wave amplitude of the female undergraduates was established as follows: for a 95% CI of multivariate reference range,Depth≥0.035 521 6. MethodsMultivariate reference range for ECG obtained in this study allows for a comprehensive utilization of the ECG data and can help the clinical diagnosis using ECG.