\[Abstract\]ObjectiveTo assess the feasibility of the single-port laparoscopic transvesical prostatectomy in pigs, and to summarize the technique difficulties, so as to lay a foundation for future clinical application.MethodsSix uncastrated male pigs were used in this study. The skin stomas were made through bladder exstrophy and the TriPort system was inserted into the bladder. Radical prostatectomy was performed as did in human using laparoscopic instruments through the TriPort system after inflating the bladder. The bladder and abdomen were closed after vesicourethral anastomosis, and the prostate specimens were removed through the skin stoma.ResultsThe first three procedures failed due to inappropriate positioning of TriPort system, limited space of bladder, and the high tension of vesicourethral anastomosis. The other 3 procedures were successful, with the periods used being 190 min,160 min, and 110 min. The whole prostates were resected completely, and the completeness of the urinary tract was recovered using interrupted suture (6 sutures).ConclusionThe single-port laparoscopic transvesical prostatectomy is a feasible procedure, but there are technical difficulties. Further experiments are needed on human cadavers before clinical application.