\[Abstract\]ObjectiveTo summarize our experience and operative techniques of transumbilical laparo-endoscopic single-site partial nephrectomy (TLSPN). MethodsTLSPN was performed on two patients for the right kidneys in August and September of 2009. ResultsThe operation was successfully done in both cases, with an additional trocar of 5 mm to retract the liver. The operation time was 255 min and 240 min; the time periods of renal artery occlusion were 48 min and 40 min; and the intraoperative blood losses were 100 ml and 50 ml. None of the patients received blood transfusion or antalgic. The drainage was removed 9 days and 5 days after operation, and the postoperative hospital stays were 13 and 12 days. ConclusionTLSPN is a safe and effective method with less trauma. However, special instruments are needed for the procedure, and the suture and knotting are somewhat difficult. An assistant trocar is needed for the procedure for the right kidney.