Objective: To observe the change of serum testosterone (T)、serum corticosterone (C) and T/C of in SD rats which have been trained in different hypoxic concentration, and provide a eligible experiment evidence for hypoxia training. Methods: 60 SD rats(8-week-old) were randomly divided into 6 groups which denominated with control (C), exercise(E), 15% control (15C), 15% exercise(15E), 11% control(11C), and 11% exercise(11E) group, the way of exercise is treadmill running , and the level of serum T and C were checked by ELISA. Results: (1) the level of T in group 11C is lower than in group C（P<0.05）; the level of T in group 11E is lower than in group E（P<0.01）; (2) the level of C in group E is higher than in group C（P<0.05）; the level of C in group 11E and 15E are lower than in group E（P<0.05）; (3) the ratio of T/C in group 11C is lower than in group C（P<0.05）. Conclusion: The 11% oxygen concentration may cause negative effects on the T/C balance, but exercise may compensate the negative effect to some extent; the 15% oxygen concentration not yet influence the T/C balance.