Objective To prepare an absorbable crosslinked microporous hemostatic starch(ACMHS), to observe its hemostasis effect in the liver, kidney and femoral artery, and to discuss the hemostasis mechanism. Methods Thirty adult healthy New Zealand rabbits of either sex, weighing 2,000-2,250 g, were equally randomized into 3 groups: liver bleeding group (group A), kidney bleeding group (group B) and femoral artery bleeding group (group C). Each group was further divided into a test group and a control group randomly (n=5) according to different hemostatic powders; ACMHS was used in the test group(group A1, B1, C1), and AristaTM was used in the control group (group A2, B2, C2). The bleeding amount and bleeding time were accurately recorded. The pathological changes at incision and in the surrounding tissues of the liver, kidney and femoral artery were observed by hematoxylin-eosin staining after test. Results All the bleeding amounts of group A1 and A2 were (1.02±0.10) g and (1.03±0.09) g, and the bleeding time periods of group A1 and A2 were (92.6±5.16) s and (95.6±5.31) s, respectively (P>0.05). All the bleeding amounts of group B1 and B2 were (0.92±0.09) g and (0.94±0.08) g, and the bleeding time periods of group B1 and B2 were (87.5±7.48) s and (88.8±6.54) s, respectively (P>0.05).All the hemostatic efforts failed in group C. Hematoxylin-eosin staining showed accumulation of red blood cells at the incision and in surrounding tissues of the liver and kidney in group A and group B, without evidence of burn. Conclusion ACMHS has the same hemostatic function as AristaTM in parenchymatous organ injury, but it is not suitable for bleeding of femoral artery injury with high pressure.