Objective: To investigate the effects of edaravone on the physiopathology and cytokine levels, such as tumor necosis factor-α (TNF-α) and interleukin-6 (IL-6), after HS in rats. Methods: 24 rats were divided into 3 groups randomly: the sham-operated control group (Sham), the haemorrhage shock goup (I/R), and the haemorrhage shock with edaravone injection (I/R-ED). Rats in the latter two groups were bled from a femoral artery catheter in 10 minutes with MAP maintained within the range of 35 to 45 mmHg for 60 minutes and resuscitaton was induced by reinjecting 60% autologous blood and 2 times shed blood Ringer’s. At the initiation and ending of resuscitation, I/R group and I/R-ED group were given the same dose (2ml/kg) of saline and edaravone, respectively. Two hours and 24 hours after the ending of resuscitation, plasma TNF-α, IL-6, ALT, AST, LDH, CK, BUN and Cr were measured separately. Results: The three groups of plasma TNF-α, IL-6, ALT, AST, LDH, CK, BUN levels is different (p<0.01) in the trend of variation with time. At 24h after resuscitation, compared with I/R group, plasma TNF-α, IL-6, GPT, GOT, LDH, CK, BUN and Cr were significantly lower in I/R-ED group (p<0.05). Conclusion: Our study suggests that edaravone could effectively protect vital organs from reperfusion injury caused by free radicals following haemorrhage shock by reducing plama levels of TNF-α and IL-6.